Purified Rabbit anti-DLX5 Antibody

SKU: DL90346A

Size 1: 100 ul

Synonyms : Homeobox protein DLX-5

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, C, Z

Applications: WB, IHC, IF/IC, IP

Background: Rabbit polyclonal antibody to DLX5

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DLX5. The exact sequence is proprietary.

WB description: Western blot analysis of Gamma-enolase expression in U937 (A), K562 (B), Jurkat (C), NIH3T3 (D), KNRK (E), HepG2 (F), HeLa (G) whole cell lysates.

IHC description: Immunohistochemical analysis of Gamma-enolase staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Gamma-enolase staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), FC (1/100 - 1/200)

Gene ID(Human): 2026

Gene ID (Mouse): 13807

Gene ID(Rat): 100911625; 24334

SwissProt (Human): P09104

SwissProt (Mouse): P17183

SwissProt (Rat): P07323

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.