Purified Rabbit anti-DHFR Antibody

SKU: DL95924A

Size 1: 100 ul

Synonyms : Dihydrofolate reductase

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC

Background: Rabbit polyclonal antibody to DHFR

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human DHFR

WB description: Western blot analysis of DHFR expression in Hela (A), HL60 (B), THP1 (C), mouse liver (D) whole cell lysates.

IHC description: Immunohistochemical analysis of DHFR staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200)

Gene ID(Human): 1719

Gene ID (Mouse): 13361

Gene ID(Rat): 24312

SwissProt (Human): P00374

SwissProt (Mouse): P00375

SwissProt (Rat): Q920D2

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.