Purified Rabbit anti-DFF45 Antibody

SKU: DL94684A

Size 1: 100 ul

Synonyms : DFF1; DFF45; DNA fragmentation factor subunit alpha; DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC

Background: Rabbit polyclonal antibody to DFF45

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DFF45. The exact sequence is proprietary.

WB description: Western blot analysis of DFF45 expression in MCF7 (A), A549 (B), H9C2 (C), CT26 (D) whole cell lysates.

IHC description: Immunohistochemical analysis of DFF45 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100)

Gene ID(Human): 1676

Gene ID (Mouse): 13347

Gene ID(Rat):

SwissProt (Human): O00273

SwissProt (Mouse): O54786

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.