Purified Rabbit anti-DAP Antibody

SKU: DL96789A

Size 1: 100 ul

Synonyms : DAP1; Death-associated protein 1; DAP-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to DAP

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human DAP

WB description: Western blot analysis of DAP expression in HepG2 (A), Hela (B), NIH3T3 (C), rat testis (D) whole cell lysates.

IHC description: Immunohistochemical analysis of DAP staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of DAP staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/20 - 1/100)

Gene ID(Human): 1611

Gene ID (Mouse): 223453

Gene ID(Rat): 64322

SwissProt (Human): P51397

SwissProt (Mouse): Q91XC8

SwissProt (Rat): Q9QX67

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.