Purified Rabbit anti-Cyclin A1/2 Antibody

SKU: DL91335A

Size 1: 100 ul

Synonyms : CCNA1; Cyclin-A1; CCNA2; CCN1; CCNA; Cyclin-A2; Cyclin-A

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, Rb

Applications: WB, IHC, IF/IC, ChIP

Background: Rabbit polyclonal antibody to Cyclin A1/2

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cyclin A1/2. The exact sequence is proprietary.

WB description: Western blot analysis of TBX15/18 expression in Jurkat (A), HepG2 (B), LOVO (C) whole cell lysates.

IHC description: Immunohistochemical analysis of TBX15/18 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of TBX15/18 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Gene ID(Human): 9096

Gene ID (Mouse): 76365; 21384

Gene ID(Rat):

SwissProt (Human): O95935; Q96SF7

SwissProt (Mouse): Q9EPZ6; O70306

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.