Purified Rabbit anti-CTCF Antibody

SKU: DL95892A

Size 1: 100 ul

Synonyms : Transcriptional repressor CTCF; 11-zinc finger protein; CCCTC-binding factor; CTCFL paralog

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, ChIP

Background: Rabbit polyclonal antibody to CTCF

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human CTCF

WB description: Western blot analysis of CTCF expression in Hela (A), PC3 (B), COS7 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of CTCF staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), ChIP (1/20 - 1/100)

Gene ID(Human): 10664

Gene ID (Mouse): 13018

Gene ID(Rat): 83726

SwissProt (Human): P49711

SwissProt (Mouse): Q61164

SwissProt (Rat): Q9R1D1

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.