Purified Rabbit anti-BCL2 (pT56) Antibody

SKU: DL94963A

Size 1: 100 ul

Synonyms : Apoptosis regulator Bcl-2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, R

Applications: WB, IHC, IF/IC, IP

Background: Rabbit polyclonal antibody to BCL2 (pT56)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BCL2 (pT56). The exact sequence is proprietary.

WB description: Western blot analysis of BCL2 (pT56) expression in Jurkat (A), rat muscle (B) whole cell lysates.

IHC description: Immunohistochemical analysis of BCL2 (pT56) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of BCL2 (pT56) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Gene ID(Human): 596

Gene ID (Mouse):

Gene ID(Rat): 24224

SwissProt (Human): P10415

SwissProt (Mouse):

SwissProt (Rat): P49950

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.