Purified Rabbit anti-BCL2 Antibody

SKU: DL90095A

Size 1: 100 ul

Synonyms : Apoptosis regulator Bcl-2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC

Background: Rabbit polyclonal antibody to BCL2

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BCL2. The exact sequence is proprietary.

WB description: Western blot analysis of BCL2 expression in MCF7 (A), mouse brain (B), rat kidney (C) whole cell lysates.

IHC description: Immunohistochemical analysis of BCL2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 596

Gene ID (Mouse): 12043

Gene ID(Rat): 24224

SwissProt (Human): P10415

SwissProt (Mouse): P10417

SwissProt (Rat): P49950

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.