Purified Rabbit anti-ATP5B Antibody

SKU: DL96129A

Size 1: 100 ul

Synonyms : ATPMB; ATPSB; ATP synthase subunit beta, mitochondrial

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC

Background: Rabbit polyclonal antibody to ATP5B

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human ATP5B

WB description: Western blot analysis of ATP5B expression in SW620 (A), HepG2 (B), mouse kidney (C), mouse brain (D) whole cell lysates.

IHC description: Immunohistochemical analysis of ATP5B staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200)

Gene ID(Human): 506

Gene ID (Mouse): 11947

Gene ID(Rat): 171374

SwissProt (Human): P06576

SwissProt (Mouse): P56480

SwissProt (Rat): P10719

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.