Purified Rabbit anti-ATP5A1 Antibody

SKU: DL90085A

Size 1: 100 ul

Synonyms : ATP5A; ATP5AL2; ATPM; ATP synthase subunit alpha, mitochondrial

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC

Background: Rabbit polyclonal antibody to ATP5A1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATP5A1. The exact sequence is proprietary.

WB description: Western blot analysis of ATP5A1 expression in HEK293T (A), Raw264.7 (B), PC12 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of ATP5A1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 498

Gene ID (Mouse): 11946

Gene ID(Rat): 65262

SwissProt (Human): P25705

SwissProt (Mouse): Q03265

SwissProt (Rat): P15999

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.