Purified Rabbit anti-ATG4D Antibody

SKU: DL94445A

Size 1: 100 ul

Synonyms : APG4D; AUTL4; Cysteine protease ATG4D; AUT-like 4 cysteine endopeptidase; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IP

Background: Rabbit polyclonal antibody to ATG4D

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATG4D. The exact sequence is proprietary.

WB description: Western blot analysis of ATG4D expression in Hela (A), A549 (B), PC12 (C), CT26 (D) whole cell lysates.

IHC description: Immunohistochemical analysis of ATG4D staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/100)

Gene ID(Human): 84971

Gene ID (Mouse): 235040

Gene ID(Rat):

SwissProt (Human): Q86TL0

SwissProt (Mouse): Q8BGV9

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.