Purified Rabbit anti-APE1 Antibody

SKU: DL90058A

Size 1: 100 ul

Synonyms : APE; APE1; APEX; APX; HAP1; REF1; DNA-(apurinic or apyrimidinic site) lyase; APEX nuclease; APEN; Apurinic-apyrimidinic endonuclease 1; AP endonuclease 1; APE-1; REF-1; Redox factor-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, P

Applications: WB, IHC

Background: Rabbit polyclonal antibody to APE1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human APE1. The exact sequence is proprietary.

WB description: Western blot analysis of APE1 expression in HeLa (A), Jurkat (B), SW480 (C), A431 (D), NIH3T3 (E), mouse kidney (F), rat lung (G) whole cell lysates.

IHC description: Immunohistochemical analysis of APE1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 328

Gene ID (Mouse): 11792

Gene ID(Rat): 79116

SwissProt (Human): P27695

SwissProt (Mouse): P28352

SwissProt (Rat): P43138

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.