Purified Rabbit anti-APC Antibody

SKU: DL90057A

Size 1: 100 ul

Synonyms : DP2.5; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, R, B, C, P

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to APC

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human APC. The exact sequence is proprietary.

WB description: Western blot analysis of APC expression in HeLa (A), HEK293T (B), SW480 (C), rat testis (D) whole cell lysates.

IHC description: Immunohistochemical analysis of APC staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of APC staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 324

Gene ID (Mouse):

Gene ID(Rat): 24205

SwissProt (Human): P25054

SwissProt (Mouse):

SwissProt (Rat): P70478

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.