Purified Rabbit anti-Alpha-enolase Antibody

SKU: DL95881A

Size 1: 100 ul

Synonyms : ENO1L1; MBPB1; MPB1; Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IF/IC

Background: Rabbit polyclonal antibody to Alpha-enolase

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human Alpha-enolase

WB description: Western blot analysis of Alpha-enolase expression in PC3 (A), Ramos (B), Hela (C), THP1 (D) whole cell lysates.

IHC description:

IF/ICC description: Immunofluorescent analysis of Alpha-enolase staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IF/IC (1/20 - 1/100)

Gene ID(Human): 2023

Gene ID (Mouse): 13806; 433182

Gene ID(Rat): 24333

SwissProt (Human): P06733

SwissProt (Mouse): P17182

SwissProt (Rat): P04764

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.