Purified Rabbit anti-ACAT1 Antibody

SKU: DL90006A

Size 1: 100 ul

Synonyms : ACAT; MAT; Acetyl-CoA acetyltransferase, mitochondrial; Acetoacetyl-CoA thiolase; T2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, Mk

Applications: WB, IHC, IP

Background: Rabbit polyclonal antibody to ACAT1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ACAT1. The exact sequence is proprietary.

WB description: Western blot analysis of ACAT1 expression in HeLa (A), HEK293T (B), SP2/0 (C), H9C2 (D), THP1 (E) whole cell lysates.

IHC description: Immunohistochemical analysis of ACAT1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Gene ID(Human): 38

Gene ID (Mouse): 110446

Gene ID(Rat): 25014

SwissProt (Human): P24752

SwissProt (Mouse): Q8QZT1

SwissProt (Rat): P17764

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.