Purified Mouse anti-NEFL Antibody

SKU: DL99565A

Size 1: 100 ul

Synonyms : NF68; NFL; Neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein

host: Mouse

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H

Applications: IH

Background: Mouse monoclonal antibody to NEFL

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human NEFL. The exact sequence is proprietary.

WB description:

IHC description: Immunohistochemical analysis of NEFL staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunohistochemical analysis of NEFL staining in human hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IP description: Immunohistochemical analysis of NEFL staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

FC description: Immunohistochemical analysis of NEFL staining in human glioma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Monoclonal

Conjugation:

Buffer: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Dilution: IH (1/100 - 1/300)

Gene ID(Human): 4747

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): P07196

SwissProt (Mouse):

SwissProt (Rat):

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.