Purified Mouse anti-Cytokeratin 14 Antibody

SKU: DL99534A

Size 1: 100 ul

Synonyms : Keratin type I cytoskeletal 14; Cytokeratin-14; CK-14; Keratin-14; K14

host: Mouse

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H

Applications: WB, IH

Background: Mouse monoclonal antibody to Cytokeratin 14

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Cytokeratin 14. The exact sequence is proprietary.

WB description: Western blot analysis of Cytokeratin 14 expression in A431 (A) whole cell lysates.

IHC description: Immunohistochemical analysis of Cytokeratin 14 staining in human esophageal squamous carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunohistochemical analysis of Cytokeratin 14 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IP description: Immunohistochemical analysis of Cytokeratin 14 staining in human squamous cell lung carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Monoclonal

Conjugation:

Buffer: Mouse IgG. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/300)

Gene ID(Human): 3861

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): P02533

SwissProt (Mouse):

SwissProt (Rat):

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.