Purified Anti-Human CD16 Antibody[3G8]

SKU: GBS-F1236A

Size:1mg

Storage:Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Flow Cytometry Antibodies

Abbreviation:CD16

Target Synonym:Fc gamma receptor;Fc gamma receptor 3;FcγRIII

Research Areas:Immunology;Innate Immunity

Conjugation:Unconjugated

Host:Mouse

Species reactivity:Human

Application:FCM;Block

Usage:Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Isotype:Mouse IgG1, κ

Clonality:Monoclonal

Clone NO.:3G8

UNIProt ID:P08637;O75015;

Background:CD16 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD16 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD16 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD16 appears to be expressed later in the immune response than CD86. CD16 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Concentration:≥ 1 mg/mL

Control catalog number:

Immunogen:Recombinant Human CD16 protein

Buffer:Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.

Purification method:>98%, Protein A/G purified

Dilution:FCM 2 μg/mL(0.5×10⁶-1×10⁶ cells)

GeneID:2214;2215