Phospho-VEGFR2 (Tyr1214) Antibody , Cat. # AF0004

SKU: AF0004

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:KDR

GeneID:3791

Uniprot:P35968

Specificity:Phospho-VEGFR2 (Tyr1214) Antibody detects endogenous levels of VEGFR2 only when phosphorylated at Tyrosine 1214.

Immunogen:A synthesized peptide derived from human VEGFR2 around the phosphorylation site of Tyr1214.

Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Reactivity:Human,Mouse,Rat

Prediction:Pig,Zebrafish,Bovine,Sheep,Rabbit,Dog,Xenopus

Application:WB,IHC,ELISA(peptide)

Dilution:WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2834089

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF0004-BP

Des# Blocking Peptide:Phospho-VEGFR2 (Tyr1214) Blocking peptide

Blocking Peptide Price:$549 CAD