Phospho-PPAR gamma (Ser112) Antibody , Cat. # AF3284

SKU: AF3284

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:PPARG

GeneID:5468

Uniprot:P37231

Specificity:Phospho-PPAR gamma (Ser112) Antibody detects endogenous levels of PPAR gamma only when phosphorylated at Serine 112.

Immunogen:A synthesized peptide derived from human PPAR- gamma around the phosphorylation site of Ser112.

Storage:PBS, pH 7.4,50% glycerol.

Reactivity:Human,Mouse,Rat

Prediction:Pig,Bovine,Horse,Sheep,Rabbit,Dog

Application:WB,IHC,IF/ICC,ELISA(peptide)

Dilution:WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:200, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2834705

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF3284-BP

Des# Blocking Peptide:Phospho-PPAR gamma (Ser112) Blocking peptide

Blocking Peptide Price:$549 CAD