PE-Cy5.5 Anti-Mouse CD122/IL-2RB Antibody(5H4)

SKU: PC55-30213

Size: 50T

Target: Mouse CD122

Form: liquid

Conjugation: PE-Cy5.5

Conjugation Information: PE-Cy5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter).

Purification: 0

Purity: 0

Host: Rat

Clonality: Monoclonal

Clone ID: 5H4

Isotype: IgG2a, κ

Storage: PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)

Background: CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.

Immunogen: 0

Synonyms: IL-2R subunit beta|CD122|High affinity IL-2 receptor subunit beta|IL-2 receptor subunit beta|IL-2RB|Il2rb|Interleukin-2 receptor subunit beta|p70-75

Observed MW:

Uniprot ID: P16297

Reactivity: Mouse

Tested Application: FC

Recommended dilution: Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

image5:

Gene ID: 16185

Research Area:

Catagory: Flow cytometry Antibodies