PDH-E1 Alpha Monoclonal Antibody

SKU: BT-MCA1012

Size:100μL

Background:The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Research_area:Signal transduction; Cancer; Metabolism

Target_protein:PDHA1

applications:WB, IF, ICC

Reactivity:Human,Mouse,Rat,Bovine,Dog,Pig

Clonality:Monoclonal

Clone ID:

Host:Mouse

Isotype:IgG

Gene Symbol/ Name:Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Immunogen:The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage:-20°C for 1 year

Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Molecular weight(Da):N/A

UniProt accession:Human: P08559; Mouse: P35486; Rat: P26284

Synonyms:PDHA1; PHE1A; Pyruvate dehydrogenase E1 component subunit alpha; somatic form; mitochondrial; PDHE1-A type I

GeneID:Human: 5160; Mouse: 18597