One-step TUNEL Flow Cytometry Apoptosis Kit (Green, FITC)

SKU: E-CK-A420

Size:100Assays

Storage:This product can be stored at -20°C for 12 months with shading light.

Shipping:Gel Packs

Exp date:12 months

Category ID_I:Apoptosis and Cell Health Detection

Category ID_II:Cell Apoptosis

Category ID_III:TUNEL Assay Kits

Conjugation:

Target Species:

Species reactivity:

Application:

Usage:

Kit Type:Apoptosis/DNA Fragmentation

Clonality:

Result Type:

Detection Instrument:Flow Cytometer

Tested Sample Types:Cell samples

Assay Time:2.5-3 hours

Detection Type:Fluorometric method;Flow cytometry

Background:

Test Principle:When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with flow cytometry.

Binding Capacity:

Buffer:

Formulation:

Reconstitution:

Calculated MW: