NLK Antibody - Cat. #: CSB-PA890657ESR1HU

SKU: CSB-PA890657ESR1HU

Size :50ul

Clone Number:

Aliases:DKFZp761G1211 antibody; FLJ21033 antibody; LAK1 antibody; Nemo like kinase antibody; Nemo-like kinase antibody; Nlk antibody; NLK_HUMAN antibody; Protein LAK1 antibody; Serine/threonine protein kinase NLK antibody; Serine/threonine-protein kinase NLK antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:Q9UBE8

Immunogen:Recombinant Human Serine/threonine-protein kinase NLK protein (278-527AA)

Raised in:Rabbit

Species Reactivity:Human

Tested Applications:ELISA, IHC; Recommended dilution: IHC:1:20-1:200

Background:Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members. Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed:25512613).

Clonality:Polyclonal

Isotype:IgG

Purification Method:Antigen Affinity Purified

Conjugate:Non-conjugated

Buffer:PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form:Liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:NLK

Research Areas:Epigenetics and Nuclear Signaling; Stem cells