Mouse Tumor Necrosis Factor Αlpha, TNF-A ELISA Kit

SKU: E0117Mo

Size:96 Tests-1 Kit

Background:Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed: 25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Research_area:Immunology; Signal Transduction; Cancer; Microbiology

Applications:ELISA

Target_protein:TNFA

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:2.49ng/L

Detection range:5-1000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Tumor Necrosis Factor alpha

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P06804

MASS(Da):25,896

GeneID:21926

Synonyms:Cachectin;C-domain 1;C-domain 2;ICD1;ICD2;Intracellular domain 1;Intracellular domain 2;N-terminal fragment;NTF;TNF A;TNF alpha;TNFA;TNF-a;TNF-alpha;Tumor necrosis factor;Tumor necrosis factor ligand superfamily member 2;Tumor necrosis factor, membrane form;Tumor necrosis factor, soluble form

Gene names:TNFA

Standard solution:1280ng/L