Mouse Semaphorin-3E (SEMA3E) ELISA Kit

SKU: AE20181MO

Size: 96T. Other sizes are also available.

Species Reactivity: Mouse (Mus musculus)

UniProt: P70275

Abbreviation: SEMA3E

Alternative Names: KIAA0331; M-SEMAH; M-SemaK; SEMAH; coll-5; semaphorin 3E

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.059 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate SEMA3E in samples. An antibody specific for SEMA3E has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA3E present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3E is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3E bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: SEMA3E encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.The deduced 775-amino acid protein has a calculated molecular mass of about 95 kD. SEMA3E shares 87.4% identity with mouse Sema3e. RT-PCR detected highest expression in brain and prostate, followed by small intestine, thymus, and lung. Little to no expression was detected in other tissues examined.Sema3e is highly expressed in developing somites, where it acts as a repulsive cue for plexin-D1-expressing endothelial cells of adjacent intersomitic vessels. Sema3e-plexin-D1 signaling did not require neuropilins, which had been presumed to be obligate Sema3 coreceptors.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).