Mouse Protein Lin-28 Homolog B, LIN28B ELISA Kit

SKU: E0247Mo

Size:96 Tests-1 Kit

Background:Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA. Does not act on pri-miR21. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation. This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent. Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop (By similarity). Mediates MYC-induced let-7 repression (PubMed:19211792). When overexpressed, may stimulate growth of carcinoma cell lines (By similarity).

Research_area:Epigenetics and Nuclear Signaling

Applications:ELISA

Target_protein:LIN28B

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:0.07ng/ml

Detection range:0.1-300ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Protein lin-28 homolog B

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q45KJ6

MASS(Da):26,903

GeneID:380669

Synonyms:2810403D23Rik;D030047M17Rik;Lin-28.2;LIN28B;Lin-28B;Protein lin-28 homolog B

Gene names:Lin28b

Standard solution:32ng/ml