Mouse OX(Orexin) ELISA Kit

SKU: ELK4723

Size:96T

Reactivity:Mouse

Alternative Names:HCRT; PPOX; Orexin A; Hypocretin 1; Orexin B; Hypocretin 2; Preproorexin; Hypocretin Neuropeptide Precursor

Assay Type:Competitive Inhibition

Sensitivity:2.74 pg/mL

Standard:500 pg/mL

Detection range:7.82-500 pg/mL

Sample type:serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Neuro science;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse OX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OX in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:O55241