Mouse MEPE(Matrix Extracellular Phosphoglycoprotein) ELISA Kit

SKU: ELK3641

Size:96T

Reactivity:Mouse

Alternative Names:OF45; Asarm Motif-Containing; Osteoblast/Osteocyte Factor 45

Assay Type:Sandwich

Sensitivity:0.21 ng/mL

Standard:30 ng/mL

Detection range:0.47-30 ng/mL

Sample type:Serum, plasma and other biological fluids.

Assay length:3.5h

Research Area:Tumor immunity;Bone metabolism;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MEPE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MEPE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MEPE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MEPE in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q8K4L6