Mouse ISG15(Interferon Stimulated Gene 15) ELISA Kit

SKU: ELK0397

Size:96T

Reactivity:Mouse

Alternative Names:G1P2; IFI15; ISG15; Interferon Alpha-Inducible Protein clone IFI-15K; Interferon Induced Protein 15; Interferon-induced 17 kDa protein; Ubiquitin Cross Reactive Protein; UCRP

Assay Type:Sandwich

Sensitivity:22.4 pg/mL

Standard:5000 pg/mL

Detection range:78.13-5000 pg/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Cytokine;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ISG15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ISG15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ISG15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ISG15 in the samples is then determined by comparing the OD of the samples to the standard curve.

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