Mouse FAM3D(Protein FAM3D) ELISA Kit

SKU: ELK0087

Size:96T

Reactivity:Mouse

Alternative Names:Oncoprotein-induced protein 1; Protein EF-7; Oit1; EF7; OIT1; cytokine-like protein EF-7; family with sequence similarity 3; family with sequence similarity 3 member D

Assay Type:Sandwich

Sensitivity:0.12 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Metabolic pathway;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FAM3D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FAM3D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FAM3D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FAM3D in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P97805