Mouse Cyclin-dependent kinase 7,CDK-7 ELISA KIT

SKU: E0966Mo

Size:96 Tests-1 Kit

Background:Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition.

Research_area:

Applications:ELISA

Target_protein:Cdk7

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:1.16mIU/ml

Detection range:2.5-160mIU/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Cyclin-dependent kinase 7

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q03147

MASS(Da):38968

GeneID:12572

Synonyms:Cdk7 Cak Cdkn7 Crk4 Mo15 Mpk-7

Gene names:Cdk7

Standard solution:320mIU/ml