Mouse CNCbl(Cyanocobalamin) ELISA Kit

SKU: ELK9671

Size:96T

Reactivity:Mouse

Alternative Names:VB12; Vitamin B12; Cobalamin; α-5,6-Dimethylbenzimidazolyl Cobamidcyanide

Assay Type:Sandwich

Sensitivity:50.9 pg/mL

Standard:10000 pg/mL

Detection range:156.25-10000 pg/mL

Sample type:serum, plasma, tissue homogenates and other biological fluids

Assay length:3.5h

Research Area:Metabolic pathway;Nutrition metabolism;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CNCbl. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CNCbl. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CNCbl, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CNCbl in the samples is then determined by comparing the OD of the samples to the standard curve.

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