Mouse a1M(Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit

SKU: ELK5551

Size:96T

Reactivity:Mouse

Alternative Names:AMBP; UTI; HCP; EDC1; HI30; IATIL; ITILC; ITI; ITIL; Alpha 1 Microglobulin/Bikunin Precursor; Growth-inhibiting protein 19; Uristatin; Uronic-Acid-Rich Protein; Trypstatin

Assay Type:Competitive Inhibition

Sensitivity:0.97 µg/mL

Standard:200 µg/mL

Detection range:3.13-200 µg/mL

Sample type:serum, plasma and other biological fluids

Assay length:2h

Research Area:Infection immunity;Immune molecule;Kidney biomarker;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse a1M. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse a1M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse a1M in the samples is then determined by comparing the OD of the samples to the standard curve.

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