KL ELISA kit (Human)

SKU: SEH757Hu

Size: 96Tests

# of Times Cited in literature: 16

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: KL

Target Full Name: Klotho

Alternative Names: KLa; Klotho Alpha

Target Species: Human

Uniprot: Q9UEF7

Gene ID: 9365

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human KL / Klotho

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.156-10ng/mL

Sensitivity: 0.055ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Klotho (KL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Klotho (KL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Klotho (KL). No significant cross-reactivity or interference between Klotho (KL) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Klotho (KL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Klotho (KL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Klotho (KL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Klotho (KL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Metabolic pathway;

References Citing This Product: High phosphate feeding induced arterial medial calcification in uremic rats: Roles of Lanthanum carbonate on protecting vasculature

Les biomarqueurs des calcifications vasculaires

Effect of secondary hyperparathyroidism serum on endothelial cells and intervention with Klotho

Human Preosteoblastic Cell Culture from a Patient with Severe Tumoral Calcinosis-Hyperphosphatemia Due to a NewGALNT3

Soluble Klotho protein as a novel serum biomarker in patients with acromegaly

Is there a role for newer biomarkers in chronic kidney disease-mineral and bone disorder management?

Clinical implication of alterations in serum Klotho levels in patients with type 2 diabetes mellitus and its associated complications

The role of klotho in chronic kidney disease

Effects of salt intervention on serum levels of Klotho influenced by salt sensitivity

Increased Plasma Level of Longevity Protein Klotho as a Potential Indicator of Cognitive Function Preservation in Patients With Schizophrenia

Investigation of the Role of Induced Overexpression of the Isolated Secreted Klotho on the A-172 Human Glioblastoma Cells

Expression of Klotho in Follicles of Patients With Polycystic Ovary Syndrome and Its Clinical Significance

Klotho is a prognostic indicator of oocytes qualities in polycystic ovary syndrome