Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro is a protein-DNA interaction assay kit specifically designed for Illumina high-throughput sequencing platforms. The Cleavage Under Targets & Tagmentation (CUT&Tag) technology is a new method to investigate protein-DNA interactions. It uses an antibody to direct a transposase fused with protein A/G to the target protein to cleave DNA near the protein binding site. With an optimized reaction system and library preparation workflow, this kit offers multiple advantages over conventional chromatin immunoprecipitation followed by sequencing (ChIP-seq), including low cell input amount, short turnaround time, high signal-to-noise ratio, and good repeatability, making it well suited for research on early embryonic development, stem cells, cancer, and epigenetics. All reagents provided in the kit have undergone strict quality control and function validation to ensure the stability and repeatability of library preparation.
This product is intended for studying protein-DNA interactions in mammalian cells with 10 - 100,000 input cells. Plant tissues and cells should be treated before proceeding with the assay using this kit.