GeneBio Systems

Hyp ELISA kit (General species)

Name: Hyp ELISA kit (General species)
Brand: GeneBio Systems
SKU: CEA621Ge
Price: 1381.00 USD
Availability: InStock

SKU:CEA621Ge

$1,381.00 USD

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Quantity

Size: 96Tests

# of Times Cited in literature: 18

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: Hyp

Target Full Name: Hydroxyproline

Alternative Names: (2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N)

Target Species: General species

Uniprot: -

Gene ID: -

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with General species Hyp / Hydroxyproline

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 61.7-5,000ng/mL

Sensitivity: 28.5ng/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hydroxyproline (Hyp) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hydroxyproline (Hyp) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Hydroxyproline (Hyp). No significant cross-reactivity or interference between Hydroxyproline (Hyp) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hydroxyproline (Hyp) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hydroxyproline (Hyp) and unlabeled Hydroxyproline (Hyp) (Standards or samples) with the pre-coated antibody specific to Hydroxyproline (Hyp). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hydroxyproline (Hyp) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hydroxyproline (Hyp) in the sample.

Research Area: Metabolic pathway;Nutrition metabolism;

References Citing This Product: Spleen-Derived Interleukin-10 Downregulates the Severity of High-Fat Diet-Induced Non-Alcoholic Fatty Pancreas Disease

Prediction of fibrosis in hepatitis C patients: assessment using hydroxyproline and oxidative stress biomarkers

Royal jelly affects collagen crosslinking in bone of ovariectomized rats

Tanshinone IIA ameliorates bleomycin-induced pulmonary fibrosis and inhibits transforming growth factor-beta-β–dependent epithelial to mesenchymal transition

Theophylline, an old drug with multi-faceted effects: Its potential benefits in immunological liver injury in rats

Effects of 4% Icodextrin on Experimental Spinal Epidural Fibrosis

Celecoxib attenuates depressive-like behavior associated with immunological liver injury inC57BL/6 mice through TNF-α and NF-κb dependent mechanisms.

Sustained-released mixture of vascular endothelial growth factor 165 and fibrin glue strengthens healing of ileal anastomoses in a rabbit model with …

Effects of panax notoginseng saponin on the pathological ultrastructure and serum IL‐6 and IL‐8 in pulmonary fibrosis in rabbits

Renin–angiotensin system inhibition ameliorates CCl-induced liver fibrosis in mice through the inactivation of nuclear transcription factor kappa B

Pirfenidone suppresses bleomycin‑induced pulmonary fibrosis and periostin expression in rats