HVD3(25-Hydroxyvitamin D3) ELISA Kit

SKU: ELK7890

Size:96T

Reactivity:General

Alternative Names:25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D

Assay Type:Competitive Inhibition

Sensitivity:0.91 ng/mL

Standard:200 ng/mL

Detection range:3.13-200 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:2h

Research Area:Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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