Human XPF(Xeroderma Pigmentosum, Complementation Group F) ELISA Kit

SKU: ELK5033

Size:96T

Reactivity:Human

Alternative Names:ERCC4; RAD1; Excision Repair Cross-Complementing Rodent Repair Deficiency,Complementation 4; DNA repair protein complementing XP-F cells; DNA excision repair ERCC-4

Assay Type:Sandwich

Sensitivity:0.06 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:Tissue homogenates, cell lysates and other biological fluids.

Assay length:3.5h

Research Area:Developmental science;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human XPF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human XPF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human XPF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human XPF in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q92889