Human Tyrosine-protein kinase ABL1 (ABL1) ELISA Kit

SKU: AE23238HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: P00519

Abbreviation: ABL1

Alternative Names: RP11-83J21.1; ABL; JTK7; bcr/abl; c-ABL; p150; v-abl; bcr/c-abl oncogene protein|proto-oncogene tyrosine-protein kinase ABL1|v-abl Abelson murine leukemia viral oncogene homolog 1

Application: ELISA

Range: 25-1600 pg/mL

Sensitivity: 6.25 pg/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate ABL1 in samples. An antibody specific for ABL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Abelson murine leukemia viral oncogene homolog 1 also known as ABL1 is a protein that, in humans, is encoded by the ABL1 gene (previous symbol ABL) located on chromosome 9. c-Abl is sometimes used to refer to the version of the gene found within the mammalian genome, while v-Abl refers to the viral gene

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).