Human TrkA/NTRK1(High affinity nerve growth factor receptor) ELISA Kit

SKU: ELK9487

Size:96T

Reactivity:Human

Alternative Names:NTRK1; MTC; TRK; TRKA; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A;

Assay Type:Sandwich

Sensitivity:32 pg/mL

Standard:5000 pg/mL

Detection range:78.13-5000 pg/mL

Sample type:serum, plasma and other biological fluids

Assay length:3.5h

Research Area:Cell Biology; Neuroscience; Signal Transduction; Developmental Biology

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TrkA/NTRK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TrkA/NTRK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TrkA/NTRK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TrkA/NTRK1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P04629