Human TOMM20(Mitochondrial Import Receptor Subunit TOM20 Homolog) ELISA Kit

SKU: ELK0255

Size:96T

Reactivity:Human

Alternative Names:MOM19; MAS20; TOM20; Mitochondrial 20 kDa outer membrane protein; Outer mitochondrial membrane receptor Tom20

Assay Type:Sandwich

Sensitivity:0.052 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:serum, plasma, tissue homogenates and other biological fluids

Assay length:3.5h

Research Area:Enzyme & Kinase;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TOMM20. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TOMM20. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TOMM20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TOMM20 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q15388