Human THBS3(Thrombospondin 3) ELISA Kit

SKU: ELK5166

Size:96T

Reactivity:Human

Alternative Names:TSP3

Assay Type:Sandwich

Sensitivity:0.59 ng/mL

Standard:100 ng/mL

Detection range:1.57-100 ng/mL

Sample type:Plasma.

Assay length:3.5h

Research Area:Hematology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human THBS3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human THBS3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human THBS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human THBS3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P49746