Human TFIIH basal transcription factor complex helicase XPD subunit,ERCC2 ELISA KIT

SKU: E1605Hu

Size:96 Tests-1 Kit

Background:ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers. {ECO:0000269|PubMed:10024882, ECO:0000269|PubMed:15494306, ECO:0000269|PubMed:20797633, ECO:0000269|PubMed:8413672}.

Research_area:

Applications:ELISA

Target_protein:ERCC2

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.017ng/ml

Detection range:0.05-10ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:TFIIH basal transcription factor complex helicase XPD subunit

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P18074

MASS(Da):86909

GeneID:2068

Synonyms:ERCC2 XPD XPDC

Gene names:ERCC2

Standard solution:12.8ng/ml