Human Tb4Y(Thymosin Beta 4, Y-Chromosomal) ELISA Kit

SKU: ELK4395

Size:96T

Reactivity:Human

Alternative Names:TMSB4Y; Thymosin,Beta 4,Y-Linked

Assay Type:Competitive Inhibition

Sensitivity:42.5 ng/mL

Standard:10000 ng/mL

Detection range:156.25-10000 ng/mL

Sample type:Serum, plasma and other biological fluids

Assay length:2h

Research Area:Endocrinology;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Tb4Y. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Tb4Y. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Tb4Y in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:O14604