Human Serine, Threonine-protein Kinase Plk3, PLK3 ELISA Kit

SKU: E3749Hu

Size:96 Tests-1 Kit

Background:Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates HIF1A and JUN is response to hypoxia. Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Also involved in Golgi disassembly during the cell cycle: part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1. May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1.

Research_area:Others

Applications:ELISA

Target_protein:PLK3

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.022ng/ml

Detection range:0.05-10ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Serine/Threonine-Protein Kinase Plk3

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q9H4B4

MASS(Da):71,629

GeneID:1263

Synonyms:Cytokine-inducible serine/threonine-protein kinase;FGF-inducible kinase;PLK 3;PLK3;PLK-3;Polo-like kinase 3;Proliferation-related kinase;Serine/threonine-protein kinase PLK3

Gene names:PLK3

Standard solution:12.8ng/ml