Human Rho-associated Protein Kinase 1, ROCK1 ELISA Kit

SKU: E3773Hu

Size:96 Tests-1 Kit

Background:Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity (PubMed: 10436159, PubMed: 10652353, PubMed: 11018042, PubMed: 11283607, PubMed: 17158456, PubMed: 18573880, PubMed: 19131646, PubMed: 8617235, PubMed: 9722579). Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A (PubMed: 10436159, PubMed: 10652353, PubMed: 11018042, PubMed: 11283607, PubMed: 17158456, PubMed: 18573880, PubMed: 19131646, PubMed: 8617235, PubMed: 9722579, PubMed: 23093407, PubMed: 23355470). Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing (PubMed: 18694941). Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress (PubMed: 19036714). Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability (By similarity). Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation (PubMed: 19181962). Required for centrosome positioning and centrosome-dependent exit from mitosis (By similarity). Plays a role in terminal erythroid differentiation (PubMed: 21072057). Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1 (By similarity). Promotes keratinocyte terminal differentiation (PubMed: 19997641). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles (By similarity).

Research_area:Others

Applications:ELISA

Target_protein:ROCK1

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.022ng/ml

Detection range:0.05-20ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Rho-Associated Protein Kinase 1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q13464

MASS(Da):158,175

GeneID:6093

Synonyms:p160 ROCK-1;p160ROCK;Renal carcinoma antigen NY-REN-35;Rho-associated protein kinase 1;Rho-associated, coiled-coil-containing protein kinase 1;Rho-associated, coiled-coil-containing protein kinase I;ROCK 1;ROCK1;ROCK-I;

Gene names:ROCK1

Standard solution:24ng/ml