Human Pyruvate kinase PKM,PKM ELISA KIT

SKU: E0894Hu

Size:96 Tests-1 Kit

Background:Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP (PubMed:15996096, PubMed:1854723). The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production (PubMed:15996096, PubMed:1854723). The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival (PubMed:15996096, PubMed:1854723). In addition to its role in glycolysis, also regulates transcription (PubMed:18191611, PubMed:21620138). Stimulates POU5F1-mediated transcriptional activation (PubMed:18191611). Promotes in a STAT1-dependent manner, the expression of the immune checkpoint protein CD274 in ARNTL/BMAL1-deficient macrophages (By similarity). Also acts as a translation regulator for a subset of mRNAs, independently of its pyruvate kinase activity: associates with subpools of endoplasmic reticulum-associated ribosomes, binds directly to the mRNAs translated at the endoplasmic reticulum and promotes translation of these endoplasmic reticulum-destined mRNAs (By similarity). Plays a general role in caspase independent cell death of tumor cells (PubMed:17308100). {ECO:0000250|UniProtKB:P52480, ECO:0000269|PubMed:15996096, ECO:0000269|PubMed:17308100, ECO:0000269|PubMed:18191611, ECO:0000269|PubMed:1854723, ECO:0000269|PubMed:21620138}.

Research_area:

Applications:ELISA

Target_protein:PKM

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.045ng/ml

Detection range:0.1-40ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Pyruvate kinase isozymes M1;M2

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P14618

MASS(Da):57937

GeneID:5315

Synonyms:PKM OIP3 PK2 PK3 PKM2

Gene names:PKM

Standard solution:48ng/ml