Human Programmed Cell Death Protein 1, PDCD1 ELISA Kit

SKU: E3331Hu

Size:96 Tests-1 Kit

Background:Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed: 21276005). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed: 21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (By similarity). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed: 28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed: 28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed: 22658127, PubMed: 25034862, PubMed: 25399552).

Research_area:Cell Biology

Applications:ELISA

Target_protein:PDCD1

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.12ng/ml

Detection range:0.2-60ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Programmed Cell Death Protein 1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q15116

MASS(Da):31,647

GeneID:5133

Synonyms:CD antigen CD279;hPD-1;PDCD 1;PDCD1;Programmed cell death protein 1;Protein PD-1

Gene names:PDCD1

Standard solution:64ng/ml