Human PITX1(Pituitary homeobox 1) ELISA Kit

SKU: ELK10073

Size:96T

Reactivity:Human

Alternative Names:Pituitary homeobox 1;PITX1;Hindlimb-expressed homeobox protein backfoot;Homeobox protein PITX1;Paired-like homeodomain transcription factor 1;BFT,;PTX1

Assay Type:Sandwich

Sensitivity:0.095 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay length:3.5h

Research Area:Immunology;Cancer

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PITX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PITX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PITX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PITX1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P78337